Application
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.Application
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/mL.Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 μg/mL.| Product Name | Orai1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Orai1Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Transmembrane protein142A Calcium releaseactivated calcium channel protein 1 Calcium release-activated calcium channel protein 1 Calcium releaseactivated calcium channel protein1 ORAI1 Transmembrane protein 142A TMEM142A |
|---|---|
| Target | Orai1 |
| Tissue Specificity | This antibody is specific for ORAI1 |
Application
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 ug/ml in the (A) absence or (B) presence of blocking peptide.
Application
Immunofluorescence of ORAI1 in Human Spleen cells with ORAI1 antibody at 20 ug/mL.
Application
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 μg/mL.| Positive Control | Blocking Peptide: Cat.No. GTX85057-PEP - ORAI1 Peptide Lysates: Cat. No. GTX27921 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
