Application
Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 ug/ml.Application
Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 ug/ml.Application
Immunofluorescence of ORAI3 in A20 cells with ORAI3 antibody at 10 ug/mL.| Product Name | ORAI3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ORAI3Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Calcium release-activated ORAI3 Calcium releaseactivated ORAI3 Calcium releaseactivated TMEM142C Transmembrane protein 142C ORAI3 Transmembrane protein142C Calcium release-activated Calcium release activated |
|---|---|
| Target | ORAI3 |
| Tissue Specificity | This antibody is specific for ORAI3 C-Terminus |
Application
Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 ug/ml.
Application
Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 ug/ml.
Application
Immunofluorescence of ORAI3 in A20 cells with ORAI3 antibody at 10 ug/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
