| Product Name | ORC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [7F6/1] to ORC1DNA replication in Saccharomyces cerevisiae requires the action of a multisubunit complex of six proteins known as the origin recognition complex (ORC). The identification of higher eukaryotic homologs of several ORC components suggests a universal role for this complex in DNA replication. ORC1, a sub-unit of the Origin Recognition Complex (ORC), is involved in the initiation of DNA replication. In human cells expression of ORC1 has been shown to be low in quiescent cells, but dramatically induced upon stimulation of cell growth. In contrast, expression of the HsOrc2 gene does not appear to be similarly regulated. HsORC1, a recently identified human protein related to yeast Orc1p and Cdc6p / Cdc18p, may be a component of the replication initiation complex in human cells. |
| Immunogen | Full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F6/1 |
| Host Species | Mouse |
| Tested Applications | WB |
| Western blotting: use at a concentration of 1 / 1250 - 1 / 2500. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 120 kDa, which corresponds to the predicted molecular weight of ORC 1.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | HSORC1 ORC1L 4998 ORC1 Q13415 PARC1 601902 |
|---|---|
| Target | ORC1 |
| Cellular Localization | Nuclear. |
| Positive Control | HeLa cells |
|---|---|
| Application Notes | Western blotting: use at a concentration of 1 / 1250 - 1 / 2500. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 120 kDa, which corresponds to the predicted molecular weight of ORC 1.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 1% BSA and 0.09% sodium azide |
