Application
Paraffin embedded sections of Kaposi’s sarcoma tissue were incubated with anti-ORF73/HHV8 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)Application
Transfected and non-transfected 293T cell lysates were resolved by SDS-PAGE, transferred to NC membrane and probed with anti- ORF73/HHV8 (1:1,000) and anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HR| Product Name | ORF73/HHV8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT4C11] to ORF73/HHV8ORF73 protein of human herpesvirus 8(HHV-8) is a kaposi's sarcoma-associated herpesvirus (kSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin and stays on the chromosomes during cell division. It maintains the viral genomes during cell division by tethering the viral episomes to the chromosomes. It binds directly to replication origin recognition complexes (ORCs) that are primarily associated with the terminal repeat (TR) region of the HHV-8 genome. |
| Immunogen | Recombinant human ORF73/HHV8 (122-329aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AT4C11 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| Western blot is 1:1,000 ~ 1:2,000 : |
|
| Species Reactivity | Kaposi's sarcoma-associated herpesvirus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Kaposi's sarcomaassociated herpesvirus Human herpesvirus 8 ORF73 Kaposi's sarcoma-associated herpesvirus Human herpesvirus8 ORF73 |
|---|---|
| Target | ORF73/HHV8 |
| Light Chain Type | kappa |
Application
Paraffin embedded sections of Kaposi’s sarcoma tissue were incubated with anti-ORF73/HHV8 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Application
Transfected and non-transfected 293T cell lysates were resolved by SDS-PAGE, transferred to NC membrane and probed with anti- ORF73/HHV8 (1:1,000) and anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HR| Application Notes | Western blot is 1:1,000 ~ 1:2,000 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
