Application
Western Blot: p14ARF Antibody [ - Detection of Human p14ARF by western blot. samples: whole cell lysate (25ug) from BT549 positive control. U2os (negative control) and p14ARF transfected IMR90 cells. Affinity purified rabbit anti p14ARF used at a 1ug/ml.| Product Name | p14ARF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Anti - p14ARF polyclonalThe INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF. |
| Immunogen | A synthetic peptide to human p14ARF (internal sequence). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | ARF CDK4I CDKN2 CMM2 INK4 INK4A MLM MTS-1 MTS1 P14 P14ARF P16 P16-INK4A P16INK4 P16INK4A P19 P19ARF TP16 MTS 1 P16 INK4A |
|---|---|
| Target | p14ARF |
| Tissue Specificity | This antibody is specific for p14ARF. |
Application
Western Blot: p14ARF Antibody [ - Detection of Human p14ARF by western blot. samples: whole cell lysate (25ug) from BT549 positive control. U2os (negative control) and p14ARF transfected IMR90 cells. Affinity purified rabbit anti p14ARF used at a 1ug/ml.| Positive Control | BT549 or HeLa whole cell extracts |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
