Application
Wild-type (WT) and p14ARF knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
p14ARF antibody detects p14ARF protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p14ARF protein stained by p14ARF antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
p14ARF antibody detects p14ARF protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of p14ARF protein from HeLa whole cell extracts using 5 μg of p14ARF antibody . Western blot analysis was performed using p14ARF antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | p14ARF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p14ARF (cyclin-dependent kinase inhibitor 2A)This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human p14ARF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cyclin-dependent kinase inhibitor 2A P16INK4 TP16 CDKN2 MTS1 P16 P14ARF CDKN2A CMM2 P16INK4A MLM ARF INK4 P19ARF P19 INK4A P16-INK4A CDK4I P14 MTS-1 |
|---|---|
| Target | p14ARF |
| Molecular Weight(MW) | 17 kDa (note) |
Application
Wild-type (WT) and p14ARF knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
p14ARF antibody detects p14ARF protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p14ARF protein stained by p14ARF antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
p14ARF antibody detects p14ARF protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of p14ARF protein from HeLa whole cell extracts using 5 μg of p14ARF antibody . Western blot analysis was performed using p14ARF antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
