WB
WB analysis using CDKN2A/P16-INK4A Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with P16-INK4A antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IHC
IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using CDKN2A/P16-INK4A Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using CDKN2A/P16-INK4A antibody at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | CDKN2A/P16-INK4A Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human P16-INK4A protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:2000-1:8000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDKN2A |
|---|---|
| Gene Synonyms | ARF MLM P14 P16 P19 CAI2 CMM2 INK4 MTS1 TP16 CDK4I CDKN2 INK4A MTS-1 P14ARF P19ARF P16INK4 P16INK4A |
| Gene Full Name | cyclin dependent kinase inhibitor 2A |
| Gene Summary | This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012] |
| Alternative Names | p16 p16 INK4 P16 INK4A p16-INK4 p16INK4A |
| Molecular Weight(MW) | 16 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
WB analysis using CDKN2A/P16-INK4A Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with P16-INK4A antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using CDKN2A/P16-INK4A Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using CDKN2A/P16-INK4A antibody at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:5000 IHC:1:2000-1:8000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
