Application
IHC Image using - Detection of p12ARF by IHC in Human Cercival Cancer| Product Name | p14ARF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to p14ARFp14 ARF arrests the cell cycle in a p53-dependent manner. p14 ARF binds to mdm 2 and promotes the rapid degradation of mdm 2 protein required for mdm 2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1 beta-encoded N-terminal domain of p14 ARF and a C-terminal region of mdm 2. Deletion of the ARF-INK 4a locus simultaneously impairs both the INK 4a-cyclin D/cdk 4-RB and the ARF-mdm 2-p53 pathways. |
| Immunogen | Synthetic peptide corresponding to the C-terminal region of Human p14ARF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FIHC-PWB |
| Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dliution of 1/50 - 1/100 in an ABC method. Immunofluorescence: assay dependent. Western blot: assay dependent. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. In Western blot, this antibody detects a band of 14 kD, which corresponds to the predicted molecular weight of p14 ARF.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | ARF CDK4I CDKN2 CMM2 INK4 INK4A MLM MTS-1 MTS1 P14 P14ARF P16 P16-INK4A P16INK4 P16INK4A P19 P19ARF TP16 MTS 1 P16 INK4A |
|---|---|
| Target | p14ARF |
Application
IHC Image using - Detection of p12ARF by IHC in Human Cercival Cancer| Positive Control | Suitable tissue control sections for Immunohistochemistry:Cervical carcinoma or breast fibroadenoma |
|---|---|
| Application Notes | Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dliution of 1/50 - 1/100 in an ABC method. Immunofluorescence: assay dependent. Western blot: assay dependent. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. In Western blot, this antibody detects a band of 14 kD, which corresponds to the predicted molecular weight of p14 ARF.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | 1% BSA and |
