Application
staining p14ARF in human lymphoma by Immunohistochemistry (FFPE-sections).Application
Human Lymphoma stained with anti-p14ARF antibody| Product Name | p14ARF antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to p14ARF predilutedThe INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Multipl p14ARF Cyclin dependent kinase inhibitor 2A TP16 Cyclin dependent kinase 4 inhibitor A MTS1 Cell cycle negative regulator beta CDKN2A CMM2 MLM Cyclin dependent kinase inhibitor2A ARF Cyclin dependent kinase inhibitor P12 Cyclin depende |
|---|---|
| Target | p14ARF |
Application
staining p14ARF in human lymphoma by Immunohistochemistry (FFPE-sections).
Application
Human Lymphoma stained with anti-p14ARF antibody| Positive Control | Hela cells, Cervical carcinoma |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
