Application
IHC Image using - Detection of p21 by IHC in Human Colon Carcinoma| Product Name | p21 Cip1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [DCS - 60.2] to p21 Cip1Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | DCS-60.2 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIP |
| Immunoprecipitation: use at 1/50.This antibody may be diluted to a titer of 1/25-1/50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAP20 CDKN1 CIP1 MDA-6 P21 SDI1 WAF1 p21CIP1 MDA 6 MDA6 |
|---|---|
| Target | p21 Cip1 |
| Tissue Specificity | This antibody recognizes a 21 kDa protein identified as p21 / WAF1 / Cip1 / Sdi1 / Pic1 tumor suppressor protein. This antibody is highly specific to p21 and does not show any cross reactivity to other mitotic inhibitors. |
| Cellular Localization | Nuclear |
Application
IHC Image using - Detection of p21 by IHC in Human Colon Carcinoma| Application Notes | Immunoprecipitation: use at 1/50.This antibody may be diluted to a titer of 1/25-1/50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide |
