Application
Immunofluorescence staining of p21 in human primary fibroblasts using anti-p21 (WA-1 ; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Application
Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1) .| Product Name | p21 Cip1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [WA - 1] to p21 Cip1Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | WA-1 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ELISA: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | CAP20 CDKN1 CIP1 MDA-6 P21 SDI1 WAF1 p21CIP1 MDA 6 MDA6 |
|---|---|
| Target | p21 Cip1 |
| Tissue Specificity | p21 protein [wild-type p53-activated fragment 1 (WAF-1) or Cdk-interacting protein (CIP1)]. |
| Cellular Localization | Nuclear |
Application
Immunofluorescence staining of p21 in human primary fibroblasts using anti-p21 (WA-1 ; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Application
Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1) .| Application Notes | ELISA: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
