| Product Name | p27 Kip1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyclonal Antibody to p27Kip1This antibody reacts with a 27 kD protein. p27 protein, also know as kinase inhibitory protein (Kip1), binds to cyclin E/cdk2 complexes. p27kip1 is a candidate tumor suppressor gene and it functions as a negative regulator of G1 progression. p27kip1 has been proposed to function as a possible mediator of TGF-b induced G1 arrest. This antibody crossreacts with mouse and rat. |
| Immunogen | A synthetic peptide derived from the C-terminal region of human p27kip1 protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDKN4 KIP1 MEN1B MEN4 P27KIP1 |
|---|---|
| Target | p27 KIP1 |
| Cellular Localization | Nuclear |
| Positive Control | Colon Carcinoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
