Application
Immunohistochemical staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody .Application
p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and blotted with p27 Kip1 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 μg of p27 Kip1 antibody . Western blot analysis was performed using p27 Kip1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (50 µg of whole cell lysate) A: MFB (mouse fibroblast) 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
p27 Kip1 antibody detects p27 Kip1 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
Sample (50 µg of whole cell lysate) A: CCRF-SB 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | p27 Kip1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Dog, Cat, Pig, Rhesus Monkey, BovineDog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDKN4 antibody KIP1 antibody MEN1B antibody MEN4 antibody P27KIP1 antibody CDKN1B antibody cyclin-dependent kinase inhibitor 1B antibody cyclin-dependent kinase inhibitor 1B (p27 Kip1) antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunohistochemical staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody .
Application
p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and blotted with p27 Kip1 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 μg of p27 Kip1 antibody . Western blot analysis was performed using p27 Kip1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (50 µg of whole cell lysate) A: MFB (mouse fibroblast) 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
p27 Kip1 antibody detects p27 Kip1 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: p27 Kip1 protein stained by p27 Kip1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
Sample (50 µg of whole cell lysate) A: CCRF-SB 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | MCF-7 , NIH-3T3 , CCRF-SB , MFB (mouse fibroblast) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
