Application
Anti-P2X4_Receptor_(extracellular) - Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), C6 cell line lysate (lanes 3 and 7) and SH-SY5Y cell line lysate (lanes 4 and 8): 1-4. Anti-P2X4 Receptor (extracellular) antibody, (1:200). 2-8. Anti-P2X4 Receptor (extracellular) antibody preincubated with the control peptide antigen.Application
Anti-P2X4_Receptor_(extracellular) - Expression of P2X4 in rat pheochromocytoma cell line (PC12) Immunocytochemical staining of intact living PC12 cells. A. Extracellular staining of cells with Anti-P2X4 (extracellular) antibody, (red, 1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI (blue). C. Merged images of A and B.| Product Name | P2X4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to P2X4ATP-activated ionotropic receptor; involved in synaptic transmission in the central nervous system [RGD, Feb 2006] |
| Immunogen | Peptide (C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 Receptor (Accession P51577). Extracellular. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P2rx4 purinergic receptor P2X ligand-gated ion channel 4 |
|---|---|
| Target | P2X4 |
Application
Anti-P2X4_Receptor_(extracellular) - Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), C6 cell line lysate (lanes 3 and 7) and SH-SY5Y cell line lysate (lanes 4 and 8): 1-4. Anti-P2X4 Receptor (extracellular) antibody, (1:200). 2-8. Anti-P2X4 Receptor (extracellular) antibody preincubated with the control peptide antigen.
Application
Anti-P2X4_Receptor_(extracellular) - Expression of P2X4 in rat pheochromocytoma cell line (PC12) Immunocytochemical staining of intact living PC12 cells. A. Extracellular staining of cells with Anti-P2X4 (extracellular) antibody, (red, 1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI (blue). C. Merged images of A and B.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
