Application
Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE diluted at 1:500Application
p53 (mono-methyl Lys372) antibody detects p53 (mono-methyl Lys372) protein at cytoplasm and nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. p53 (mono-methyl Lys372) antibody diluted at 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53 (mono-methyl Lys372) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | p53 (mono-methyl Lys372) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p53 (mono-Methyl K372) (tumor protein p53)This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq] |
| Modification | mono-methyl Lys372 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Sheep, Rhesus MonkeySheep (100%), Rhesus Monkey (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ92943 antibody LFS1 antibody P53 antibody TRP53 antibody TP53 antibody cellular tumor antigen p53 antibody antigen NY-CO-13 antibody phosphoprotein p53 antibody p53 tumor suppressor antibody transformation-related protein 53 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (8) |
| Cellular Localization | Cytoplasm , Nucleus , Endoplasmic reticulum |
Application
Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE diluted at 1:500
Application
p53 (mono-methyl Lys372) antibody detects p53 (mono-methyl Lys372) protein at cytoplasm and nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. p53 (mono-methyl Lys372) antibody diluted at 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53 (mono-methyl Lys372) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | HCT116 24hr cisplatin 30uM , 48hr cisplatin uM |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
