Application
Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive. 1A – anti-p53 (total)Application
Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive. 1B – anti-p53 (phospho Ser392)| Product Name | p53 (phospho Ser392) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [FP3-2] to human p53 ( P392 )Ser392 phosphorylation of p53 has been shown to occur following DNA damage. Phosphorylation of p53 at this site may be mediated the casein kinase 2 (CK2), and also be other phosphorylation mechanisms. |
| Immunogen | KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53. |
| Modification | p-Ser392 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | FP3-2 [FPS392] |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| For IHC-P, IP and WB: Use at an assay dependent dilution. Optimal concentrations/dilutions should be determined by the researcher. * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ92943 LFS1 P53 TRP53 p53 (phospho S392) p53 (phospho Ser392) p53 (pS392) p53 (pSer392) p53 phospho S392 p53 phospho Ser392 Phospho p53 (S392) Phospho p53 (Ser392) Phospho p53 (pS392) Phospho p53 (pSer392) |
|---|---|
| Target | p53 (Phospho S392) |
| Tissue Specificity | The antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor protein phosphorylated at CKII site (Ser 392). |
Application
Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive. 1A – anti-p53 (total)
Application
Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive. 1B – anti-p53 (phospho Ser392)| Positive Control | Colon or breast carcinoma |
|---|---|
| Application Notes | For IHC-P, IP and WB: Use at an assay dependent dilution. Optimal concentrations/dilutions should be determined by the researcher. * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | (PBS) with 0.09% sodium azide, pH 7.4 |
