Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
p53 antibody detects p53 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody diluted by 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [D01] . Western blot analysis was performed using p53 antibody [D01] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | p53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human p53The p53 antibody, clone DO1, specifically recognizes an epitope at the N-terminal end of the human p53 protein between amino acids 20-25. p53 is a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. And p53, as a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15, a mutation which reduces the ability of p53 to arrest cell growth. |
| Immunogen | Recombinant human p53 expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | D01 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-FIHC-PIPWB |
| For WB: Use at a dilution of 1:500-1:10000, For IP: Use at a dilution of 1:100-1:500 , For IHC-P: Use at a dilution of 1:1,000. Antigen retrieval can be done with EDTA at pH8.0 - Some customers have stated that the product works well without antigen retrieval. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DO1 D01 p53 DO 1 D0 1 DO-1 D0-1 53 Antigen NY CO 13 Cellular tumor antigen p53 Phosphoprotein p53 TP 53 TP53 Transformation Related Protein p53 TRP 53 TRP53 Tumor protein p53 Li Fraumeni syndrome Tumor suppressor p53 Tumour Protein p53 |
|---|---|
| Target | TP53 |
| Light Chain Type | kappa |
Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
p53 antibody detects p53 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody diluted by 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [D01] . Western blot analysis was performed using p53 antibody [D01] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HCT116 , 293T (0.5 µM doxorubicin treatment for 24 hr) , HCT116(30 μM cisplatin treatment for 24 hr) |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:10000, For IP: Use at a dilution of 1:100-1:500 , For IHC-P: Use at a dilution of 1:1,000. Antigen retrieval can be done with EDTA at pH8.0 - Some customers have stated that the product works well without antigen retrieval. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS |
