Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 µM cisplatin treatment for 24 hr 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | p53 antibody [Pab240] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human p53PAb240 specifically recognizes human p53 protein, a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. p53, a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate that an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15 is a mutation which reduces the ability of p53 to arrest cell growth. |
| Immunogen | Gel-purified p53 containing p53 amino acids 14-389 (derived from the pSV53C p53 cDNA clone). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | Pab240 |
| Host Species | Mouse |
| Tested Applications | ELISA,FACS,ICC/IF,IHC-F,IHC-P,IP,WB |
| For IHC: Use at a dilution of 1:250 - 1:500. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining. For IP: Use at 10µg/mg of lysate. By immunoprecipitation, this antibody reacts with only MUTANT p53protein under NON-DENATURING conditions. For WB: Use at a dilution of 1:2,000. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse,Dog |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ92943, LFS1, P53, TRP53 |
|---|---|
| Target | TP53 |
| Light Chain Type | kappa |
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 µM cisplatin treatment for 24 hr 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | HBL100 , HCT116 , 293T , A431 |
|---|---|
| Application Notes | For IHC: Use at a dilution of 1:250 - 1:500. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining. For IP: Use at 10µg/mg of lysate. By immunoprecipitation, this antibody reacts with only MUTANT p53protein under NON-DENATURING conditions. For WB: Use at a dilution of 1:2,000. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
