| Product Name | p53 DINP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to p53 DINP1Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible gene was identified recently and designated p53DINP1 (for p53-dependent damage-inducible nuclear protein 1) and SIP (for stress induced protein) in human and mouse (1,2). A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks (1). p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene encodes two proteins of 27 and 18 kDa in human and mouse termed p53DINP1-a and p53DINP1-b or SIP27 and SIP18 (1,2). p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress (2). |
| Immunogen | Synthetic peptide: amino acids 1-14 of human p53DINP1. This sequence is identical between alpha and beta, and differs by one amino acid from those of mouse. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dillution of 1 to 2 μg/ml. Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, and mouse liver and kidney tissue lysates. Optimal concentrations/dilutions should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434M1317 FLJ22139 SIP TP53DINP1 TP53INP1A TP53INP1B Teap p53DINP1 |
|---|---|
| Target | p53 DINP1 |
| Positive Control | Human lung tissue lysate. Also human spleen, mouse liver and kidney tissue lysates. |
|---|---|
| Application Notes | For WB: Use at a dillution of 1 to 2 μg/ml. Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, and mouse liver and kidney tissue lysates. Optimal concentrations/dilutions should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 with 0.02% sodium azide as preservative |
