Application
IHC-P analysis of colon carcinoma using p57 Kip2 antibody [57P06].Application
IHC Image using - Detection of p57 Kip2 by IHC in Human Colon Carcinoma| Product Name | p57 Kip2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to p57Kip2 ( Mitotic Inhibitor / Suppressor Protein )This antibody is specific to a protein of 57 kD known as p57Kip2 a cell cycle regulatory mitotic inhibitor. This antibody does not cross react with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes and is negative regulator of cell proliferation. |
| Immunogen | BALB/C mice were injected with mouse recombinant p57Kip2 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 57P06 |
| Host Species | Mouse |
| Tested Applications | IHC-PIP |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 min at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For IP: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Synonyms | p57Kip2 Cdkn1c 12577 AL024410 Kip2 p57(kip2) p57 Kip2 CDKI |
|---|---|
| Target | p57 Kip2 |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
IHC-P analysis of colon carcinoma using p57 Kip2 antibody [57P06].
Application
IHC Image using - Detection of p57 Kip2 by IHC in Human Colon Carcinoma| Positive Control | Colan carcinoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. We suggest an incubation period of 30 min at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For IP: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS contains 0.05% sodium azide. |
