| Product Name | p57 Kip2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [KP39] to p57 Kip2p57 / Kip 2 (or CDKN 1C) is a potent tight binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57 / Kip 2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilms' tumor, and Beckwith Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF II) and H19, both of which seem to be implicated in adrenal neoplasms. |
| Immunogen | Recombinant full length protein (Human) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | KP39 |
| Host Species | Mouse |
| Tested Applications | IHC-PIP |
| NOT suitable for Western blotting. For IP(native): Use at a concentration of 2μg/mg. For IHC-P: Use at a concentration of 2-4 μg/ml for 30 min at RT. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | BWCR BWS KIP2 WBS p57 |
|---|---|
| Target | p57 Kip2 |
| Cellular Localization | Nuclear. |
| Light Chain Type | kappa |
| Positive Control | LS174T cells. Colon carcinomas or placenta |
|---|---|
| Application Notes | NOT suitable for Western blotting. For IP(native): Use at a concentration of 2μg/mg. For IHC-P: Use at a concentration of 2-4 μg/ml for 30 min at RT. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
