Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p70 S6K antibody diluted at 1:50000.Application
p70 S6K antibody detects p70 S6K protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p70 S6K protein stained by p70 S6K antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.Application
p70 S6K antibody detects p70 S6K protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung carcinoma. p70 S6K antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:10000WB
TAC operation for 2 weeks markedly induced activation of AKT, mTOR, S6K, and ERK1/2 and inhibition of AMPK activation compared with sham controls, but this effect was reversed by RES in TAC-treated mice (D).| Product Name | p70 S6K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to S6K (ribosomal protein S6 kinase, 70kDa, polypeptide 1)This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p70 S6K. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:1000 IHC-p:1:50~1:100 ICC/IF:1:100~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rabbit, Chicken, BovineXenopus laevis (87%), Rabbit (99%), Chicken (96%), Bovine (99%) |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | PS6K antibody S6K antibody S6K1 antibody STK14A antibody p70(S6K)-alpha antibody p70-S6K antibody p70-alpha antibody RPS6KB1 antibody ribosomal protein S6 kinase I antibody p70 S6 kinase alpha 1 antibody serine/threonine kinase 14 alpha antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (4) |
| Cellular Localization | Cytoplasm (By similarity) , Cell junction , synapse , synaptosome |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p70 S6K antibody diluted at 1:50000.
Application
p70 S6K antibody detects p70 S6K protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p70 S6K protein stained by p70 S6K antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Application
p70 S6K antibody detects p70 S6K protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung carcinoma. p70 S6K antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:10000
WB
TAC operation for 2 weeks markedly induced activation of AKT, mTOR, S6K, and ERK1/2 and inhibition of AMPK activation compared with sham controls, but this effect was reversed by RES in TAC-treated mice (D).| Positive Control | Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | WB:1:500~1:1000 IHC-p:1:50~1:100 ICC/IF:1:100~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
