Application
PAD4 antibody detects PAD4 protein at cytoplasm on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. PAD4 antibody diluted at 1:500.Application
PAD4 antibody detects PAD4 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PADI4 antibody immunoprecipitates PADI4 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of PADI4 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAD4 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4 antibody at 1:100 dilution.| Product Name | PAD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAD4 (peptidyl arginine deiminase, type IV)This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PAD antibody PAD4 antibody PADI5 antibody PDI4 antibody PDI5 antibody PADI4 antibody peptidyl arginine deiminase type V antibody PADI-H protein antibody protein-arginine deiminase type IV antibody HL-60 PAD antibody protein-arginine deiminase ty |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , Cytoplasmic granule |
Application
PAD4 antibody detects PAD4 protein at cytoplasm on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. PAD4 antibody diluted at 1:500.
Application
PAD4 antibody detects PAD4 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PADI4 antibody immunoprecipitates PADI4 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of PADI4 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAD4 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4 antibody at 1:100 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
