Application
PAF49 antibody [GT635] detects CD3EAP protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE PAF49 antibody [GT635] dilution: 1:1000Application
CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: CD3EAP protein stained by CD3EAP antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody [GT635] diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.| Product Name | PAF49 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT635] to PAF49 (CD3e molecule, epsilon associated protein)DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT635 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 55 kDa (note) (2) |
|---|---|
| Cellular Localization | Nucleus , nucleolus |
Application
PAF49 antibody [GT635] detects CD3EAP protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE PAF49 antibody [GT635] dilution: 1:1000
Application
CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: CD3EAP protein stained by CD3EAP antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody [GT635] diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , A549 , H1299 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
