Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PAICS monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS.Application
Anti-PAICS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PAICSApplication
HEK293T cells transfected with either RC215225 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PAICS antibody, and then analyzed by flow cytometry.| Product Name | PAICS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8A1] to PAICSThis gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 8A1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC1343 MGC5024 PAIS ADE2 ADE2H1 PAICS AIRC DKFZp781N1372 |
|---|---|
| Target | PAICS |
| Tissue Specificity | is specific for human PAICS (NP_006443). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PAICS monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS.
Application
Anti-PAICS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PAICS
Application
HEK293T cells transfected with either RC215225 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PAICS antibody, and then analyzed by flow cytometry.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
