Application
Immunofluorescent imaging of human cells (U2OS) with reveals the expected highly specific membranous distribution. IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised wApplication
Madin Darby Bovine Kidney (MDBK) extract was separated on SDS-PAGE and probed with Monoclonal AB to PAN Cadherin (MO) CL: CH-19 (PREPK) (Cat. No. ). The antibody was developed with HRP APA Mouse Fab ads/HIgG conjugate and a NBT/BCIP substrate. Antibody dilution 1/2,000| Product Name | pan Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CH - 19] to pan Cadherin - Plasma Membrane MarkerThis product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type. |
| Immunogen | Synthetic peptide: DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponding to amino acids 889-912 of Chicken N-Cadherin (cdh2). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CH-19 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| IF: Use at a dilution of 1/500 using cultured MDBK cells. IHC-P: Use at a dilution of 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseChickenDogPigRatCatGoatGuinea PigHamsterRabbitSandratSheepSnakeBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | pan Cadherin |
|---|---|
| Tissue Specificity | Reacts with all cadherins. |
| Cellular Localization | Cell membrane. |
Application
Immunofluorescent imaging of human cells (U2OS) with reveals the expected highly specific membranous distribution. IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised w
Application
Madin Darby Bovine Kidney (MDBK) extract was separated on SDS-PAGE and probed with Monoclonal AB to PAN Cadherin (MO) CL: CH-19 (PREPK) (Cat. No. ). The antibody was developed with HRP APA Mouse Fab ads/HIgG conjugate and a NBT/BCIP substrate. Antibody dilution 1/2,000| Application Notes | IF: Use at a dilution of 1/500 using cultured MDBK cells. IHC-P: Use at a dilution of 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid with 0.1% sodium azide |
