| Product Name | pan Cytokeratin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [B311.1] to pan CytokeratinThis antibody is a broad spectrum antibody and reacts with several normal and neoplastic epithelia. This antibody reacts with simple epithelium and both suprabasal and basal layers of cornifying and noncornifying squamous epithelium. It also works in smears of cultured epithelial cell lines. |
| Immunogen | Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | B311.1 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-PIPWB |
| Western Blotting 1:100-1:400This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100. High temperature antigenic unmasking technique or enzymatic predigestion is recommended with this antibody when using paraffin-embedded tissue sections.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | pan Cytokeratin |
|---|---|
| Tissue Specificity | This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond to cytokeratins 4,5,6,8,10,13 and 18. |
| Positive Control | Skin, Colonic Carcinoma |
|---|---|
| Application Notes | Western Blotting 1:100-1:400This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100. High temperature antigenic unmasking technique or enzymatic predigestion is recommended with this antibody when using paraffin-embedded tissue sections.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 1% BSA and 0.09% sodium azide |
