Application
Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11) .Application
Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using anti-cytokeratin antibody (C-11) PE. Overlay with Isotype mouse IgG1 control (PPV-06).| Product Name | pan Cytokeratin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [C-11] to pan Cytokeratin |
| Immunogen | A keratin-enriched preparation from human epidermoid carcinoma cell line A431 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | C-11 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| FACS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseMammalian species |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | pan Cytokeratin |
|---|---|
| Tissue Specificity | Cytokeratin peptides 4,5,6,8,10,13,18 |
Application
Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11) .
Application
Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using anti-cytokeratin antibody (C-11) PE. Overlay with Isotype mouse IgG1 control (PPV-06).| Application Notes | FACS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 0.09% sodium azide |
