Mouse Monoclonal antibody [AE1/AE3] to pan-CytokeratinIs a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins. It produces positive staining in virtually all epithelia.
Immunogen
BALB/C mice were immunized with human epidermal keratin.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AE1/AE3
Host Species
Mouse
Tested Applications
IHCIHC-P
For IHC-P: Use at a concentration of 1:25-1:50 (ABC method). We suggest an incubation period of 30 minutes at room temperature. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
HumanMouse
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Target
pan-Cytokeratin
Application
Application
IHC Image using - Detection of Cytokeratin pan by IHC in Human Tonsil
Positive Control
Squamous lung carcinoma
Application Notes
For IHC-P: Use at a concentration of 1:25-1:50 (ABC method). We suggest an incubation period of 30 minutes at room temperature. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.