Application
IHC Image using - Detection of Cytokeratin pan by IHC in Human TonsilApplication
Formalin fixed paraffin embedded human tonsil stained with Cytokeratin (AE1 & AE3) antibody (cat. # )| Product Name | pan-Cytokeratin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Cytokeratin AE1 / AE3 |
| Immunogen | BALB/C mice were immunized with human epidermal keratin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AE1/AE3 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| IHC-P : 1:50-1:100. Antigen retrieval buffer : Citrate Buffer pH 6.0. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Cytokeratin pan |
|---|---|
| Tissue Specificity | This AE1/AE3 antibody cocktail recognizes most of the acidic (Type I) keratins as well as basic (Type II). It recognizes keratin polypeptides of 40, 48, 50, 52, 54, 56.5, 58, 59 and 64 to 67 kD. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers, where as 58 kD keratin is present in the basal and suprabasal layers, while 65 to 67 kD keratin is present in the cells above the basal layers. This antibody can be used to stain distinct cell layers in an epithelial tissue and various neoplasms of epithelial origin. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | Kappa & kappa |
Application
IHC Image using - Detection of Cytokeratin pan by IHC in Human Tonsil
Application
Formalin fixed paraffin embedded human tonsil stained with Cytokeratin (AE1 & AE3) antibody (cat. # )| Positive Control | Squamous lung carcinoma |
|---|---|
| Application Notes | IHC-P : 1:50-1:100. Antigen retrieval buffer : Citrate Buffer pH 6.0. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH7.2, containing 0.05% sodium azide |
