Application
Formalin fixed paraffin embedded human tonsil stained with Cytokeratin (AE1 & AE3) antibody (cat. # )| Product Name | pan-Cytokeratin antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [AE1/AE3] to pan-CytokeratinIs a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins. It produces positive staining in virtually all epithelia. |
| Immunogen | Human epidermal keratins |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AE1/AE3 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| For IHC-P: Use at a neat dilution. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. IHC-Fr: Use at a neat dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Target | pan-Cytokeratin |
|---|---|
| Tissue Specificity | Please see Background/Relevance information |
Application
Formalin fixed paraffin embedded human tonsil stained with Cytokeratin (AE1 & AE3) antibody (cat. # )| Positive Control | Squamous lung carcinoma |
|---|---|
| Application Notes | For IHC-P: Use at a neat dilution. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. IHC-Fr: Use at a neat dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate Buffered Saline containing 1% BSA and 0.09% sodium azide |
