Application
ICC/IF ImageFetal rat lung epithelial cells were labeled with Monoclonal Anti-pan-Cytokeratin (mixture) (green) and DNA was counterstained with DAPI (blue).| Product Name | pan-Cytokeratin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [mixture] to pan-CytokeratinIntermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral-to-basic subfamily.Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti-Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. |
| Immunogen | Prepared from a mixture of human cytokeratins. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | mixture |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| IHC-P: Use at a dilution of 1/100. WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | RatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | pan-Cytokeratin |
|---|---|
| Tissue Specificity | recognizes human cytokeratins 1, 4, 5, 6, 8, 10, 13, 18, and 19 in immunoblotting. It is a broad spectrum reagent which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudo-stratified epithelia. It does not react with non-epithelial normal human tissues. |
Application
ICC/IF ImageFetal rat lung epithelial cells were labeled with Monoclonal Anti-pan-Cytokeratin (mixture) (green) and DNA was counterstained with DAPI (blue).| Application Notes | IHC-P: Use at a dilution of 1/100. WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Whole Serum, 7% Horse serum, 10ug/ml Phenol red |
