Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PARP1 antibody [N2C1], Internal detects PARP1 protein by western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated) B. 30 µg HCT116 whole cell lysate/extract (30 µM cisplatin treatment for 24hr) 7.5% SDS-PAGE PARP1 antibody [N2C1], Internal dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP protein stained by PARP antibody [N2C1], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.Application
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.Application
PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 30 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody [N2C1], Internal dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PARP antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PARP (poly (ADP-ribose) polymerase 1)This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADPRT antibody ADPRT 1 antibody ADPRT1 antibody PARP antibody PARP-1 antibody PPOL antibody pADPRT-1 antibody PARP1 antibody poly[ADP-ribose] synthase 1 antibody poly(ADP-ribosyl)transferase antibody poly(ADP-ribose) polymerase antibody poly(ADP |
|---|---|
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PARP1 antibody [N2C1], Internal detects PARP1 protein by western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated) B. 30 µg HCT116 whole cell lysate/extract (30 µM cisplatin treatment for 24hr) 7.5% SDS-PAGE PARP1 antibody [N2C1], Internal dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP protein stained by PARP antibody [N2C1], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.
Application
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
Application
PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 30 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody [N2C1], Internal dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , HCT116 , HCT116 (30uM cisplatin treatment for 24hr) , NIH-3T3 , PC-12 , *H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
