Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] diluted at 1:1000.Application
PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE PARP1 antibody [GT6212] dilution: 1:1000Application
PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated) B. 30 µg HCT116 whole cell lysate/extract (30µM Cisplatin treatment for 24hr) 7.5 % SDS-PAGE PARP1 antibody [GT6212] dilution: 1:1000Application
PARP antibody [GT6212] detects PARP protein at nucleus in human Hep G2 by immunohistochemical analysis. Sample: Paraffin-embedded human Hep G2. PARP antibody [GT6212] diluted at 1:250.| Product Name | PARP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to PARP (poly (ADP-ribose) polymerase 1)This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT6212 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (90%), Rat (88%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADPRT antibody ADPRT 1 antibody ADPRT1 antibody PARP antibody PARP-1 antibody PPOL antibody pADPRT-1 antibody PARP1 antibody poly[ADP-ribose] synthase 1 antibody poly(ADP-ribosyl)transferase antibody poly(ADP-ribose) polymerase antibody poly(ADP |
|---|---|
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT6212] diluted at 1:1000.
Application
PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5 % SDS-PAGE PARP1 antibody [GT6212] dilution: 1:1000
Application
PARP1 antibody [GT6212] detects PARP1 protein by Western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated) B. 30 µg HCT116 whole cell lysate/extract (30µM Cisplatin treatment for 24hr) 7.5 % SDS-PAGE PARP1 antibody [GT6212] dilution: 1:1000
Application
PARP antibody [GT6212] detects PARP protein at nucleus in human Hep G2 by immunohistochemical analysis. Sample: Paraffin-embedded human Hep G2. PARP antibody [GT6212] diluted at 1:250.| Positive Control | 293T , HCT116 (untreated) , HCT116 (30µM Cisplatin treatment for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
