Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PTCH1 mouse monoclonal antibody.Application
Anti-PTCH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PTCH1Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PTCH1 mouse monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.| Product Name | Patched1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5c7] to Patched1Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 122-436 of human Patched1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 5c7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PTCH1 NBCCS PTC HPE7 PTC1 patched BCNS |
|---|---|
| Target | Patched1 |
| Tissue Specificity | is specific for amino acids 122-436 of human Patched1 |
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Anti-PTCH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PTCH1
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PTCH1 mouse monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.| Application Notes | IHC 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
