Application
Paxillin (phospho Tyr118) antibody [GT162] detects Paxillin (phospho Tyr118) protein at focal adhesion sites by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin (phospho Tyr118) protein stained by Paxillin (phospho Tyr118) antibody [GT162] diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] diluted at 1:500.Application
Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] diluted at 1:3000.| Product Name | Paxillin (phospho Tyr118) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT162] to Paxillin (phospho Tyr118) (paxillin) |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Paxillin (phospho Tyr118). The exact sequence is proprietary. |
| Modification | p-Tyr118 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT162 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ChickenMouse (100%), Rat (100%), Chicken (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PXN antibody Paxillin antibody |
|---|---|
| Target | Paxillin |
| Molecular Weight(MW) | 65 kDa (note) (3) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell junction , focal adhesion |
Application
Paxillin (phospho Tyr118) antibody [GT162] detects Paxillin (phospho Tyr118) protein at focal adhesion sites by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin (phospho Tyr118) protein stained by Paxillin (phospho Tyr118) antibody [GT162] diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] diluted at 1:500.
Application
Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] diluted at 1:3000.| Positive Control | A431 (1 mM pervanadate, 37ºC treatment for 30 min) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
