Application
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether. Fixed cells were stained with 1:200 Monoclonal Anti-Paxillin Clone: PXC-10 . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.Application
Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Paxillin Clone: PXC-10 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. A431 4. JURKAT 5. NIH-3T3| Product Name | Paxillin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PXC - 10] to PaxillinCellular adhesion to the extracellular matrix is critically involved in many processes including normal and transformed cell growth, migration, metastasis, lymphocyte extravasation and also in force transmission during muscle contraction. Paxillin (68 kDa), is a cytoskeletal component found in the focal adhesions at the ends of actin stress fibers, but not in adherens junctions of the cells. Paxillin interacts with several proteins including members of the src family of tyrosine kinases, the transforming protein v-crk, the cytoskeletal protein vinculin, and the tyrosine kinase, focal adhesion kinase (FAK). This interaction has suggested a function for paxillin as a molecular adaptor, responsible for the recruitment of structural and signaling molecules to focal adhesions. The paxillin molecule has a single binding site for vinculin, and at least two binding sites for FAK, that are separated by an intervening sequence of 100 amino acids. Upon transformation by RSV, 20-30% of the paxillin molecules are phosphorylated on tyrosine. In addition, the cellular distribution of paxillin becomes more diffuse, suggesting that phosphorylation of paxillin may have a role in the disassembly of focal adhesions and stress fibers during transformation. The availability of monoclonal antibody reacting specifically with paxillin enables the subcellular detection and localization of paxillin. |
| Immunogen | Recombinant fragment (amino acids 305-559 of Chicken Paxillin). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | PXC-10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB: Use at a dilution of 1/500. Detects bands of approximately 40 and 68 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRatHamsterBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Paxillin |
|---|---|
| Cellular Localization | Cytoplasmic |
Application
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Polyethylene glycol tert-octylphenyl ether. Fixed cells were stained with 1:200 Monoclonal Anti-Paxillin Clone: PXC-10 . The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Application
Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Paxillin Clone: PXC-10 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. A431 4. JURKAT 5. NIH-3T3| Application Notes | WB: Use at a dilution of 1/500. Detects bands of approximately 40 and 68 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide |
