Application
Human Tonsil stained with anti-PCNA antibody| Product Name | PCNA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PCNAPCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Immunogen | Recombinant human PCNA protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| IHC-P: Use at a dilution of 1/200 for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cyclin DNA polymerase delta auxiliary protein MGC8367 Proliferating Cell Nuclear Antigen |
|---|---|
| Target | PCNA |
| Cellular Localization | Nuclear |
Application
Human Tonsil stained with anti-PCNA antibody| Positive Control | Tonsil, lymph node, or small intestine |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/200 for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
