Application
Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNAApplication
Staining of KM-H2 cells with MOUSE ANTI PCNA:FITC following permeabilisation.| Product Name | PCNA antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PC10] to PCNA-Proliferation Marker predilutedPCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Immunogen | Rat PCNA made in the protein A expression vector pR1T2T. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | PC10 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-FIHC-PWB |
| This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature.However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high tempersture antigen unmasking with 10mM citrate buffer,pH6.0 prior to immunostaining: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | Cyclin DNA polymerase delta auxiliary protein MGC8367 Proliferating Cell Nuclear Antigen |
|---|---|
| Target | PCNA |
| Tissue Specificity | PCNA is essential for cellular DNA synthesis. This antibody reacts with proliferating cells in a wide range of normal tissues. The significance of PCNA immunoreactivity in tumors in not fully established yet. However, in non-Hodgkin's lymphoma, a linear relation between Ki-67 and PCNA staining has been demonstrated. |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA
Application
Staining of KM-H2 cells with MOUSE ANTI PCNA:FITC following permeabilisation.| Application Notes | This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature.However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high tempersture antigen unmasking with 10mM citrate buffer,pH6.0 prior to immunostaining: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.05% sodium azide |
