Application
ELISA analysis of control antigen and antigen using PCNA antibody [7H4F8].Application
FACS analysis of MOLT4 cells using PCNA antibody [7H4F8] (green) or a negative control (purple).Application
IHC-P analysis of cervical cancer tissues using PCNA antibody [7H4F8].Application
IHC-P analysis of colon cancer tissues using PCNA antibody [7H4F8].Application
WB analysis of (1) A431, (2) HEK293, (3) HeLa, (4) HepG2, (5) Raji, (6) MOLT4, (7) COS7 and (8) MCF-7 lysates using PCNA antibody [7H4F8].Application
WB analysis of lysates from (1) untransfected and (2) PCNA (a.a. 53-196)-hIgGFc transfected HEK293 cells using PCNA antibody [7H4F8].Application
WB analysis of human PCNA recombinant protein using PCNA antibody [7H4F8].| Product Name | PCNA antibody (ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [7H4F8] to PCNAThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 53 and 196 of human PCNA expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7H4F8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Target | PCNA |
|---|---|
| Molecular Weight(MW) | 28.7 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PCNA antibody [7H4F8].
Application
FACS analysis of MOLT4 cells using PCNA antibody [7H4F8] (green) or a negative control (purple).
Application
IHC-P analysis of cervical cancer tissues using PCNA antibody [7H4F8].
Application
IHC-P analysis of colon cancer tissues using PCNA antibody [7H4F8].
Application
WB analysis of (1) A431, (2) HEK293, (3) HeLa, (4) HepG2, (5) Raji, (6) MOLT4, (7) COS7 and (8) MCF-7 lysates using PCNA antibody [7H4F8].
Application
WB analysis of lysates from (1) untransfected and (2) PCNA (a.a. 53-196)-hIgGFc transfected HEK293 cells using PCNA antibody [7H4F8].
Application
WB analysis of human PCNA recombinant protein using PCNA antibody [7H4F8].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
