Application
Immunostaining of PDE2A expressing cells with (Ab dilution 1:250)Application
Western blot using in antibody dilution buffer| Product Name | PDE2A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE2ACyclic nucleotides are important intracellular second messengers which play an important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodiesterases. One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is PDE2. Two genes, PDE2A and PDE2B, have been identified and cloned and they both have an apparent molecular weight of 100 kDA. PDE2A has specificity for cAMP and its activity is modulated by the presence of cGMP. In contrast, PDE2B does not hydrolyze cGMP nor its activity is altered by the presence of cGMP. Western blot analyses of PDE2A reveals that it is present in a variety of tissues including neocortex, cerebellum, heart, kidney lungs, pulmonary artery and skeletal muscle. |
| Immunogen | The immunogen corresponding to a region within amino acids 909 and 926 of human PDE2A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIPWB |
|
For WB: Use at a dilution of 1:250-1:1000. : |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | O00408 5138 602658 PED2A4 PDE2A PDE2A1 |
|---|---|
| Target | PDE2A |
| Cellular Localization | Cell Membrane |
Application
Immunostaining of PDE2A expressing cells with (Ab dilution 1:250)
Application
Western blot using in antibody dilution buffer| Positive Control | Rat brain |
|---|---|
| Application Notes |
For WB: Use at a dilution of 1:250-1:1000. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
