Application
Anti-PDE4A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PDE4AApplication
Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody .Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A.| Product Name | PDE4A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1E3] to PDE4AHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Immunogen | Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1E3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PDE4 DPDE2 PDE4A |
|---|---|
| Target | PDE4A |
| Tissue Specificity | is specific for human PDE4A (NP_006193). |
| Cellular Localization | Isoform 4 has propensity for association with membranes. Predominantly cytoplasmic. |
Application
Anti-PDE4A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PDE4A
Application
Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody .
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
