Application
Western blot of rat testis and heart homogenate using (1:500 dilution)| Product Name | PDE8B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE8BCyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 5-prime,3-prime-cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been grouped into several families based on substrate affinities, inhibitor sensitivities, mode of regulation, and amino acid sequence homologies. The PDE8 family contains high-affinity cAMP-specific, IBMX (3-isobutyl-1-methyl-xanthine)-insensitive PDEs. All PDEs share a conserved C-terminal catalytic region and a variable N-terminal domain that presumably accounts for the distinctive regulatory properties unique to the individual families. PDE8B exhibits cAMP PDE activity which was not inhibited by various PDE inhibitors, including IBMX. The cAMP hydrolytic activity was unaffected by cGMP, and no cGMP PDE hydrolysis has been detected. Northern blot analysis indicated that the 4.2-kb PDE8B mRNA was expressed at the highest levels in thyroid, and at lower levels in brain, spinal cord, and placenta. |
| Immunogen | Synthetic peptide (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| FuncS: Use at an assay dependent dilution. This antibody is ideal for activity measurement and screening of various PDE8B enzyme variants in sample. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADSD FLJ11212 PPNAD3 |
|---|---|
| Target | PDE8B |
| Tissue Specificity | This antibody has no cross-reactivity against PDE8A protein or any other PDE family members. |
Application
Western blot of rat testis and heart homogenate using (1:500 dilution)| Positive Control | Rat testis and heart |
|---|---|
| Application Notes | FuncS: Use at an assay dependent dilution. This antibody is ideal for activity measurement and screening of various PDE8B enzyme variants in sample. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 'Stabilization buffer' with 0.02% thimerosal or merthiolate. |
