Application
IHC Image using - Detection of PDGF beta by IHC in Human Angiosarcoma| Product Name | PDGF beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDGF betaPlatelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly and indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. |
| Immunogen | Synthetic peptide: TEVFEISRRLIDRTNA, corresponding to amino acids 101-116 of Human PDGF beta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. Incubate for 30 minutes at room temperature.ABC method: Use at a dilution of 1:50-1:100. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRatCatHamsterSheepBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | PDGF2 csis c-sis SSV Simian sarcoma viral (v-sis) oncogene homolog vsis platelet deriv Platelet derived growth factor beta polypeptide Simian sarcoma viral (vsis) oncogene homolog SIS vsis platelet derived growth factor beta polypeptide v-sis pla |
|---|---|
| Target | PDGF beta |
| Tissue Specificity | This antibody is specific for b form and reacts with PDGF (AB) and PDGF (BB) protein. |
| Cellular Localization | Cytoplasmic |
Application
IHC Image using - Detection of PDGF beta by IHC in Human Angiosarcoma| Positive Control | Angiosarcoma |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. Incubate for 30 minutes at room temperature.ABC method: Use at a dilution of 1:50-1:100. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azdie |
