Application
Western blot analysis of PDI: Lane 1: MW marker Lane 2: HeLa (HS) Lane 3: ADI-SPP-891 Lane 4: Vero (HS) Lane 5: 3T3 (HS) Lane 6: PC-12 (HS) Lane 7: CHO-K1 (HS)Application
Immunohistochemistry analysis of human HEK293 cells transfected with wild type TorsinA co-stained with PDI mAb (1D3) (red). Co-localization of TorsinA and PDI proteins merge as yellow.| Product Name | PDI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1D3] to PDI - ER MarkerThe three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| Immunogen | Peptide corresponding to amino acids 499-509 of rat protein disulfide isomerase conjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| For ICC: Use at a concentration of 2-5 μg/ml. For IP: Use at a concentration of 8-12 μg/ml. For WB: Use at a concentration of 2-4 μg/ml. Predicted molecular weight: 58 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDogMonkeyPigRatGuinea PigHamsterSheepXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Disulphide Isomerase GIT Glutathione Insulin Transhydrogenase P4HB PHDB PO4HB Procollagen Proline 2 Oxoglutarate 4 Dioxygenase beta PROHB Prolyl 4 Hydroxylase beta Protein Disulphide Isomerase Thyroid Hormone Binding Protein p55 |
|---|---|
| Target | PDI |
Application
Western blot analysis of PDI: Lane 1: MW marker Lane 2: HeLa (HS) Lane 3: ADI-SPP-891 Lane 4: Vero (HS) Lane 5: 3T3 (HS) Lane 6: PC-12 (HS) Lane 7: CHO-K1 (HS)
Application
Immunohistochemistry analysis of human HEK293 cells transfected with wild type TorsinA co-stained with PDI mAb (1D3) (red). Co-localization of TorsinA and PDI proteins merge as yellow.| Application Notes | For ICC: Use at a concentration of 2-5 μg/ml. For IP: Use at a concentration of 8-12 μg/ml. For WB: Use at a concentration of 2-4 μg/ml. Predicted molecular weight: 58 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2, containing 50% Glycerol and 0.1mM PMSF |
