| Product Name | PDI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PDI-38H8] to PDI - ER MarkerThe three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| Immunogen | Tissue / cell preparation. Yeast nuclear protein preparations. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | PDI-38H8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF: 1/250 - 1/2500. WB: 1/1000 - 1/10000. Predicted molecular weight: 57 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Concentration | 1mg/ml |
| Gene Synonyms | Disulphide Isomerase GIT Glutathione Insulin Transhydrogenase P4HB PHDB PO4HB Procollagen Proline 2 Oxoglutarate 4 Dioxygenase beta PROHB Prolyl 4 Hydroxylase beta Protein Disulphide Isomerase Thyroid Hormone Binding Protein p55 |
|---|---|
| Target | PDI |
| Tissue Specificity | recognises Protein Disulphide Isomerase (PDI) |
| Cellular Localization | Endoplasmic reticulum lumen |
| Application Notes | ICC/IF: 1/250 - 1/2500. WB: 1/1000 - 1/10000. Predicted molecular weight: 57 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: Sodium Azide; Constituents: 50% Glycerol, 1mg/ml BSA |
