Application
Western blot of p5 in HT 1080 cell lysate using| Product Name | PDIA6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDIA6P5 is a novel protein that was originally identified in humans. Functionally, P5 has been shown to have peptide binding ability and chaperone activity specific to certain proteins. P5 shares several structural similarities to PDI, an important protein of the ER. The deduced amino acid sequence of P5 shows that it contains the ER retention signal KDEL. P5 also contains two CXXC-like motifs, a motif responsible for oxidoreductase activity. These two CXXC motifs are found on the N- and C-terminus of the protein, one at each end. Studies suggest that the N-terminal CXXC motif is more important than the second for isomerase activity. Contrary to most of the PDI-like proteins, P5 is not stress-inducible. |
| Immunogen | Synthetic peptide: DIDLSDVELDDLGKDEL, corresponding to amino acids 476-492 of Human P5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB: 1:10000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMousePig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERP5 antibody P5 antibody TXNDC7 antibody |
|---|---|
| Target | PDIA6 |
| Cellular Localization | Endoplasmic reticulum |
Application
Western blot of p5 in HT 1080 cell lysate using| Positive Control | Human fibrosarcoma HT 1080 whole cell lysate |
|---|---|
| Application Notes | WB: 1:10000 Optimal dilution / concentration should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Antiserum containing 0.01% sodium azide |
