Application
Immunoprecipitation of PDIA6 protein from 293T whole cell extracts using 5 μg of PDIA6 antibody [N1N3] . Western blot analysis was performed using PDIA6 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000Application
PDIA6 antibody [N1N3] detects PDIA6 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PDIA6 antibody [N1N3] diluted at 1:500.Application
PDIA6 antibody [N1N3] detects PDIA6 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PDIA6 antibody [N1N3] diluted at 1:500.| Product Name | PDIA6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDIA6 (protein disulfide isomerase family A, member 6)Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDIA6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, Chimpanzee, BovinePig (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ERP5 antibody P5 antibody TXNDC7 antibody PDIA6 antibody protein disulfide isomerase P5 antibody protein disulfide isomerase-associated 6 antibody thioredoxin domain-containing protein 7 antibody ER protein 5 antibody endoplasmic reticulum protein |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (6) |
| Cellular Localization | Endoplasmic reticulum lumen (By similarity) , Melanosome |
Application
Immunoprecipitation of PDIA6 protein from 293T whole cell extracts using 5 μg of PDIA6 antibody [N1N3] . Western blot analysis was performed using PDIA6 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000
Application
PDIA6 antibody [N1N3] detects PDIA6 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PDIA6 antibody [N1N3] diluted at 1:500.
Application
PDIA6 antibody [N1N3] detects PDIA6 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PDIA6 antibody [N1N3] diluted at 1:500.| Positive Control | 293T , A431 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
