Mouse monoclonal antibody [AT1A2] to PDZK1This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
Immunogen
Recombinant human PDZK1 (1-519aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG2
Clone Number
AT1A2
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
WB analysis of HeLa and 293T lysates (40ug per lane) using PDZK1 antibody at a dilution of 1:1,000.
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.