Application
A549 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | PEDF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C4] to PEDFPigment epithelium-derived factor (PEDF) is a 50kDa glycoprotein and member of the serpin superfamily without any activity of serine protease. PEDF is an angiogenesis inhibitor with neurotrophic properties, balances angiogenesis in the eye and blocks tumor progression. That is, in neuronal cell, PEDF is promoting cell survival and differentiation, however, when endothelial cells are exposed to PEDF, their cell migration and proliferation are inhibited, and they undergo apoptosis. |
| Immunogen | Recombinant human PEDF (19-418 aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1C4 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:500 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Pigment epithelium derived factor Proliferation inducing protein 35 Proliferation inducing protein35 Pigment epithelium-derived factor EPC 1 SERPIN F1 PIG 35 Pigment epitheliumderived factor EPC1 EPC-1 Serpin peptidase inhibitor clade F member 1 |
|---|---|
| Target | PEDF |
| Cellular Localization | Enriched in stage I melanosomes. |
| Light Chain Type | kappa |
Application
A549 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:500 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
